The calculated distance (air line) between Prostějov and Ústí nad Labem is approximately 159 miles respectively 256 kilometers.
By car or train, the actual journey to Ústí nad Labem is certainly longer, as only the direct route (as the crow flies) between Prostějov and Ústí nad Labem has been calculated here.
Air line (approximately)
256 kilometers
138 nautical miles