The calculated distance (air line) between Colonia General Alfredo Stroessner and Ayolas is approximately 156 miles respectively 251 kilometers.
By car or train, the actual journey to Ayolas is certainly longer, as only the direct route (as the crow flies) between Colonia General Alfredo Stroessner and Ayolas has been calculated here.
Air line (approximately)
251 kilometers
136 nautical miles