The calculated distance (air line) between Tlacotepec and Santiago de Querétaro is approximately 163 miles respectively 262 kilometers.
By car or train, the actual journey to Santiago de Querétaro is certainly longer, as only the direct route (as the crow flies) between Tlacotepec and Santiago de Querétaro has been calculated here.
Air line (approximately)
262 kilometers
141 nautical miles