The calculated distance (air line) between Juanacatlán and Santiago de Querétaro is approximately 180 miles respectively 290 kilometers.
By car or train, the actual journey to Santiago de Querétaro is certainly longer, as only the direct route (as the crow flies) between Juanacatlán and Santiago de Querétaro has been calculated here.
Air line (approximately)
290 kilometers
156 nautical miles