The calculated distance (air line) between Brno and Ústí nad Labem is approximately 153 miles respectively 246 kilometers.
By car or train, the actual journey to Ústí nad Labem is certainly longer, as only the direct route (as the crow flies) between Brno and Ústí nad Labem has been calculated here.
Air line (approximately)
246 kilometers
133 nautical miles