The calculated distance (air line) between Eusebio Ayala and Itauguá is approximately 25 miles respectively 40 kilometers.
By car or train, the actual journey to Itauguá is certainly longer, as only the direct route (as the crow flies) between Eusebio Ayala and Itauguá has been calculated here.
Air line (approximately)
40 kilometers
21 nautical miles