The calculated distance (air line) between Johor Bahru and Kota Tinggi is approximately 21 miles respectively 34 kilometers.
By car or train, the actual journey to Kota Tinggi is certainly longer, as only the direct route (as the crow flies) between Johor Bahru and Kota Tinggi has been calculated here.
Air line (approximately)
34 kilometers
18 nautical miles