The calculated distance (air line) between Brandýs nad Labem-Stará Boleslav and Brno is approximately 111 miles respectively 178 kilometers.
By car or train, the actual journey to Brno is certainly longer, as only the direct route (as the crow flies) between Brandýs nad Labem-Stará Boleslav and Brno has been calculated here.
Air line (approximately)
178 kilometers
96 nautical miles