The calculated distance (air line) between Anastasiyevskaya and Rostov-na-Donu is approximately 164 miles respectively 265 kilometers.
By car or train, the actual journey to Rostov-na-Donu is certainly longer, as only the direct route (as the crow flies) between Anastasiyevskaya and Rostov-na-Donu has been calculated here.
Air line (approximately)
265 kilometers
143 nautical miles