The calculated distance (air line) between Ambérieu-en-Bugey and Besançon is approximately 95 miles respectively 152 kilometers.
By car or train, the actual journey to Besançon is certainly longer, as only the direct route (as the crow flies) between Ambérieu-en-Bugey and Besançon has been calculated here.
Air line (approximately)
152 kilometers
82 nautical miles